HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PA2G4 (Cat# RC210230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PA2G4(Cat# TA503203). Positive lysates [LY416812] (100 ug) and [LC416812] (20 ug) can be purchased separately from OriGene.