HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF4E (Cat# RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E(Cat# TA503042). Positive lysates [LY400723] (100 ug) and [LC400723] (20 ug) can be purchased separately from OriGene.