HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AURKC (RC213890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AURKC. Positive lysates LY423127 (100 ug) and LC423127 (20 ug) can be purchased separately from OriGene.