Western blot analysis of extracts of various cell lines, using pan-TriMethyl-lysine pAb (TA379602) at 1:500 dilution.HeLa and MCF7 cells were treated by ADOX (100 μM) for 24 hours .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit .|Exposure time: 3min.