Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody (TA377051) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit .|Exposure time: 60s.