WB was performed on histone extracts from HeLa cells (15ug) with the abtibody against H3K79me1, diluted 1:1,000 and 1:2000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (Bio-Rad, broad range biotinylated SDS-PAGE standard) is shown on the left, the location of the protein of interest is indicated on the right.