Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor 594) B - cell nuclei stained with DAPI (blue) C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)