Figure 1. Calcium dose-response for ghrelin stimulation of CHO/GHSR cells compared to untransfected CHO-K1 cells. The passage 1and passage 3 of CHO/GHSR cells that stably express the GHSR (Green and blue circles) or untransfected CHO-K1 cells which is the same passage as CHO/GHSR MCB (black triangle) were loaded with Fluo-4 NW (Invitrogen), then stimulated with the indicated concentration of ghrelin. The change in intracellular calcium was measured by a FlexStation III fluorescent plate reader. Data poins represent the average+/- standard deviation of triplicate determinations.
