Ordering Details
Cat Number:	P-7001-96
Size:	each
Price:	£521.00
Quantity:
Shipping:	£12.00

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Data Sheet

EpiQuik In Situ Histone H3 Phosphorylation [Ser10] Assay Kit [96 assays]

Partner: Epigentek Group Inc

Applications:	Amount Quantitation
Description:	EpiQuik In Situ Histone H3 Phosphorylation (Ser10) Assay Kit is a whole cell-based detection kit of histone H3 phosphorylation (ser10). In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The histone H3 phosphorylation (ser10) is then detected by an anti-phospho histone H3 (ser10) antibody. The ratio or amount of phospho histone H3 (ser10) can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Shipping Conditions:	Ship on cold packs
Usage:	Research Use Only

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Schematic procedure for using the EpiQuik In Situ Histone H3 Phosphorylation (Ser10) Assay Kit.

$Log-phase MCF-7 cells were serum-starved overnight, and serum was added back for 30 minutes followed by treatment with Calyculin A (CL-A, 0.2 Î¼M for 30 minutes). The level of H3 phosphorylation at ser10 was measured with this kit.$

Log-phase MCF-7 cells were serum-starved overnight, and serum was added back for 30 minutes followed by treatment with Calyculin A (CL-A, 0.2 Î¼M for 30 minutes). The level of H3 phosphorylation at ser10 was measured with this kit.