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Cat Number:P-6001-096
Size:each
Price:£498.00
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Shipping:£12.00

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EpiQuik In Situ DNA Damage Assay Kit [96 assays]

Partner: Epigentek Group Inc

Applications:Amount Quantitation
Description:EpiQuik In Situ DNA Damage Assay Kit is a whole cell-based detection of DNA damage and/or apoptosis. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The phosphorylation of H2AX at serine139, the sensitive marker of DNA damage, is then detected by an anti-phospho H2AXSer139 antibody. The ratio or amount of phospho H2AXSer139 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.
Shipping Conditions:Ship on cold packs
Usage:Research Use Only

 

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Schematic procedure for using the EpiQuik In Situ DNA Damage Assay Kit.

Schematic procedure for using the EpiQuik In Situ DNA Damage Assay Kit.

MCF-7 cells in microplate wells were exposed to etoposide for 4 h and DNA damage was detected by the measuring level of phosphor-H2AX.

MCF-7 cells in microplate wells were exposed to etoposide for 4 h and DNA damage was detected by the measuring level of phosphor-H2AX.