Applications: | ELISA, IF, IHC, WB |
Species Reactivity: | Human, Mouse, Rat, Monkey |
Isotype: | Mouse IgG1 kappa |
Immunogen: | Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development. |
Purification: | Anti-AKT pS473 Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This phospho specific monoclonal antibody is specific for phosphorylated human and mouse AKT protein at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined. |
Description: | Boster Bio Anti-Akt phospho S473 Monoclonal Antibody (Catalog # MP00024). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. |
Synonym: | mouse anti-AKT pS473, phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1 |
Shipping Conditions: | Ship on cold packs |
Storage: | Store Anti-AKT pS473 (MOUSE) Monoclonal Antibody at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Usage: | Research use only |