Data Sheet

MSDS

2X PCR SuperMix

Description:	2X PCR SuperMix is a cocktail of calculated amounts of Mg++, dNTPs, and recombinant Taq DNA Polymerase for highly efficient amplification of nucleic acid templates by the polymerase chain reaction (PCR). 2X PCR SuperMix is supplied at a 2X concentration that allows the easy calculation of its final concentration for adding the primer and template to the mixture. The supplied reagent is sufficient for 100 amplification reactions of 50 uL each. The 2X PCR SuperMix is very stable, and no detectable reduction of PCR performance or enzyme activity is observed after storage for 12 months at 4 C. Repeated freeze-thaw cycles do not reduce performance or activity.
Further Information:	Quality Control: 2X PCR SuperMix is evaluated by DNA polymerization activity assay that measures the percent of Taq DNA polymerase inhibition versus an uninhibited control. A functional assay is also performed. Components of the 2X PCR SuperMix are tested for the absence of DNase, RNase and exonuclease activities. Recombinant Taq DNA polymerase is tested for the absence of exonuclease, and double- and single-stranded endonuclease activities. The enzyme is >90% homogeneous as determined by SDS polyacrylamide gel electrophoresis.
Shipping Conditions:	Ship on freezer packs
Storage:	Stable for up to 1 year at -20 C
Usage:	Research use only