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WB analysis of whole cell (25 ug,lane 1) and histone extracts from HeLa cells,and 1 ug of recombinant histone H2A,H2B,H3 and H4 (lane 3,4,5 and 6,respectively) using H3K18ac antibody at a dilution of 1:500 in TBS-Tween containing 5% skimmed milk.