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All five standards are 426 bp with all cytosines either unmodified (lane 1,C),5-methylcytosine (lane 2,5mC),5-hydroxymethylcytosine (lane 3,5hmC),5-formylcytosin (lane4,5fC),5-carboxycytosine (lane5,5-caC). DNA marker: 1 kb Plus DNA marker.