HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NR4A3 (Cat# RC218250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR4A3(Cat# TA804893). Positive lysates [LY429326] (100 ug) and [LC429326] (20 ug) can be purchased separately from OriGene.