HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3R5 (RC222249, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3R5. Positive lysates LY402310 (100 ug) and LC402310 (20 ug) can be purchased separately from OriGene.