HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NT5DC1 (Cat# RC211087, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NT5DC1(Cat# TA501590). Positive lysates [LY403486] (100 ug) and [LC403486] (20 ug) can be purchased separately from OriGene.