All lanes : Anti-cyfip1 Antibody (Center) at 1:2000 dilutionLane 1: ZF4 whole cell lysatesLane 2: Zebrafish lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 145 kDaBlocking/Dilution buffer: 5% NFDM/TBST.