Ordering Details
Cat Number:	ATGA0434-100ul
Size:	100uL
Concentration:	1 mg/mL
Price:	£205.00
Quantity:
Shipping:	£12.00

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MSDS

S100A11 [AT20D11], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:	ELISA, WB, FACS, ICC/IF
Species Reactivity:	Human
Isotype:	Mouse IgG1 kappa
Immunogen:	Recombinant human S100A11 (1-105aa) purified from E. coli
Purification:	By protein-A affinity chromatography
Conjugation:	Unconjugated
Description:	S100A11 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in motility, invasion, and tubulin polymerization.
Further Information:	The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:	S100 calcium binding protein A11, MLN70, S100C
Ascension Number:	P31949
NCBI EntrezGene:	NP_005611
Shipping Conditions:	Ship on cold packs
Storage:	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:	Research use only

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human S100A11 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysate Lane 2. : MCF7 cell lysate Lane 3. : PC3 cell lysate Lane 4. : HaCaT cell lysate Lane 5. : THP1 cell lysate Lane 6. : U87MG cell lysate Lane 7. : A427 cell lysate Lane 8. : SK-OV-3 cell lysate Lane 9. : A549 cell lysate

ICC/IF analysis of S100A11 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human S100A11 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).