Applications: | ELISA, WB |
Species Reactivity: | Human |
Isotype: | Mouse IgG1 kappa |
Immunogen: | Recombinant human C-Myc (410-419aa) with tag purified from E. coli |
Purification: | By protein-A affinity chromatography |
Conjugation: | Unconjugated |
Description: | A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. |
Further Information: | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
Ascension Number: | P01106 |
NCBI EntrezGene: | NP_002458 |
Shipping Conditions: | Ship on cold packs |
Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Usage: | Research use only |