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Cat Number:ATGA0395-100ul
Size:100uL
Concentration:1 mg/mL
Price:£205.00
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Shipping:£12.00

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Myc tag [AT19B4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human C-Myc (410-419aa) with tag purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Ascension Number:P01106
NCBI EntrezGene:NP_002458
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The recombinant human SNCA-Myc tag protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000)Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000)Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000)The recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human SNCA-Myc tag (20ng) Lane 2. : Recombinant Human Myc-GST tag (50ng)Lane 3. : Recombinant Human s100A8-Myc tag (50ng)Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng)

The recombinant human SNCA-Myc tag protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Anti-human Myc tag monoclonal antibody (1:1000)Lane 2. : Anti-Human Myc tag monoclonal antibody (1:5000)Lane 3. : Anti-Human Myc tag monoclonal antibody (1:10000)The recombinant protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Myc tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human SNCA-Myc tag (20ng) Lane 2. : Recombinant Human Myc-GST tag (50ng)Lane 3. : Recombinant Human s100A8-Myc tag (50ng)Lane 4. : Recombinant Human CHI3L1-Myc tag (50ng)