| Applications: | ELISA, WB, ICC/IF |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2a kappa |
| Immunogen: | Recombinant human KRT20 (1-424aa) purified from E.coli. |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa. In immunohistochemistry, antibodies to KRT20 can be used to identify a range of adenocarcinoma arising from epithelia that normally contain the KRT20 protein. For example, the protein is commonly found in colorectal cancer, transitional cell carcinomas and in Merkel cell carcinoma, but is absent in lung cancer, prostate cancer, and non-mucinous ovarian cancer. It is often used in combination with antibodies to CK7 to distinguish different types of glandular tumor. |
| Further Information: | The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:3000, ICC/IF and Flow cytometry is 1:200 |
| Synonym: | Keratin type I cytoskeletal 20, Keratin, type I cytoskeletal 20, CD20; CK-20; CK20; K20; KRT21 |
| Ascension Number: | P35900 |
| NCBI EntrezGene: | NP_061883 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
