Your Basket 0

 

Ordering Details

Cat Number:ATGA0287-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
Quantity:
Shipping:£12.00

Document Links

MSDS

 

DNAL1 [AT29E4], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG2b kappa
Immunogen:Recombinant human DNAL1 (1-190aa) purified from E. coli
Purification:By protein-A affinity chromatography
Conjugation:Unconjugated
Description:DNAL1, also known as dynein light chain 1 axonemal, is component of the outer dynein arms complex. This complex acts as the molecular motor that provides the force to move cilia in an ATP-dependent manner. DNAL1 is expressed in tissues with motile cilia or flagella and may be involved in the movement of sperm flagella. Homozygous mutations in the DNAL1 gene are associated with primary ciliary dyskinesia 16 (CILD16) which is a disorder characterized by abnormalities of motile cilia.
Further Information:The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Synonym:Dynein light chain 1 axonemal, Dynein light chain 1, axonemal, C14orf168, CILD16
Ascension Number:Q4LDG9
NCBI EntrezGene:NP_113615
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

Insight Biotechnology Ltd reserves the right to change pricing without notice

 

The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates of HeLa, HepG2 and MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DNAL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of DNAL1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human      DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of DNAL1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human      DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

ICC/IF analysis of DNAL1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of DNAL1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human DNAL1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).