The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PCBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: K562 cell lysate Lane 4.: 293T cell lysate Lane 5.: Jurkat cell lysate Lane 6.: MCF7 cell lysate
ICC/IF analysis of PCBP1 in MCF-7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of PCBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PCBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
