The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human CTSBLane 2. : Recombinant Human CTSDLane 3. : Recombinant Human CTSELane 4. : Recombinant Human CTSFLane 5. : Recombinant Human CTSHLane 6. : Recombinant Human CTSKLane 7. : Recombinant Human CTSLLane 8. : Recombinant Human CTSSLane 9. : Recombinant Human CTSWLane 10. : Recombinant Human CTSZThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTSS antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : HeLa cell lysateLane 2. : U87MG cell lysateLane 3. : A549 cell lysate
ICC/IF analysis of CTSS in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of CTSS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).ICC/IF analysis of CTSS in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTSS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
