| Applications: | ELISA, WB, FACS |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG1 kappa |
| Immunogen: | Recombinant human ICT1 (30-206aa) purified from E. coli |
| Purification: | By protein-A affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. This neoplastic-induced deviation is marked by a change in expression of several mRNAs. ICT1 is a member of the prokaryotic/mitochondrial release factor family whose expression is downregulated over 4 fold upon colon stem cell differentiation. This downregulation of ICT1 could lead to its use as a marker for detection of colon carcinomas. |
| Further Information: | The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000. |
| Synonym: | Peptidyl-tRNA hydrolase ICT1 mitochondrial, Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1 |
| Ascension Number: | Q14197 |
| NCBI EntrezGene: | NP_001536 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
