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Cat Number:ATGA0148-50ul
Size:50uL
Concentration:1mg/mL
Price:£123.00
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Shipping:£12.00

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GFP [AT2G5], Mouse Monoclonal Antibody

Partner: NKMax Bio (ATGen)

Applications:ELISA, WB
Isotype:Mouse IgG2a kappa
Immunogen:Recombinant GFP (1-238aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Further Information:The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.
Synonym:Green fluorescent protein
Ascension Number:P42212
NCBI EntrezGene:P42212
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The recombinant protein GFP (20, 40 and 80ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: GFP 20ngLane 2.: GFP 40ngLane 3.: GFP 80ngThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: GFP transfected 293T cell lysate

The recombinant protein GFP (20, 40 and 80ng) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-GFP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: GFP 20ngLane 2.: GFP 40ngLane 3.: GFP 80ngThe cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GFP antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: GFP transfected 293T cell lysate