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Cat Number:ATGA0128-100ul
Size:100ul
Concentration:1 mg/ml
Price:£197.00
Quantity:
Shipping:£10.00

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Data Sheet
MSDS

 

eIF5A [AT4D1], Mouse Monoclonal Antibody

Partner: ATGen

Applications:ELISA, WB, FACS, ICC/IF
Species Reactivity:Human
Isotype:Mouse IgG1 kappa
Immunogen:Recombinant human EIF5A (1-154aa) purified from E. coli
Purification:By protein-G affinity chromatography
Conjugation:Unconjugated
Description:Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.
Further Information:The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Synonym:Eukaryotic translation initiation factor 5A isoform B, EIF-5A, EIF5A1, eIF5AI, Eukaryotic translation initiation factor 5A isoform B eIF 4D, EIF 5A, eIF 5A 1, eIF 5A1, eIF4D, uORF, uORF A, Eukaryotic initiation factor 5A, Rev binding factor, Eukaryotic initiation factor 5A isoform 1, MGC99547, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A 1, MGC104255.
Ascension Number:P63241
NCBI EntrezGene:NP_001961
Shipping Conditions:Ship on cold packs
Storage:Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Usage:Research use only

 

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The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

ICC/IF analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human   EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

ICC/IF analysis of EIF5A in Balb/3T3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human EIF5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)