The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ADRM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : MCF7 cell lysateLane 2 : HeLa cell lysateLane 3 : K562 cell lysateLane 4 : PC3 cell lysateLane 5 : 293T cell lysateLane 6 : HepG2 cell lysateLane 7 : Jurkat cell lysateLane 8 : U87MG cell lysate
ICC/IF analysis of ADRM1 in MCF7 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ADRM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).