Fluorescent image of C2C12 cell stained with BRAF Antibody (Center)(Cat#AP7810c).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor� 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37?).Cytoplasmic actin was counterstained with Alexa Fluor� 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37?).BRAF immunoreactivity is localized to Cytoplasm significantly.
