All lanes : Anti-ACTR2 Antibody (C-term) at 1:1000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.