All lanes : Anti-CEA Antibody (Center) at 1:1000 dilutionLane 1: MCF-7 whole cell lysateLane 2: HT-29 whole cell lysateLane 3: PC-3 whole cell lysateLane 4: A549 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDaBlocking/Dilution buffer: 5% NFDM/TBST.