All lanes : Anti-PIGU Antibody (N-Term) at 1:2000 dilutionLane 1: mouse heart lysateLane 2: mouse kidney lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDaBlocking/Dilution buffer: 5% NFDM/TBST.