Ordering Details
Cat Number:	AP21780b
Size:	200uL
Price:	£304.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

H3f3b [C-term] [RB53621], Rabbit Polyclonal Antibody

Partner: Abcepta, Inc.

Applications:	WB
Species Reactivity:	Mouse
Isotype:	Rabbit Ig
Purification:	Purified Rabbit Polyclonal Antibody
Description:	Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Further Information:	This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 103-136 amino acids from mouse H3f3b.
Synonym:	Histone H33, H3f3a, H33a
Ascension Number:	P84244
NCBI EntrezGene:	15078;15081
Shipping Conditions:	Ship on cold packs
Storage:	Maintain refrigerated at 2-8 C for up to 2 weeks. For long term storage store at -20 C in small aliquots to prevent freeze-thaw cycles.
Usage:	Research use only

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All lanes : Anti-H3f3b Antibody (C-Term) at 1:8000 dilutionLane 1: NIH/3T3 whole cell lysateLane 2: mouse lung lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDaBlocking/Dilution buffer: 5% NFDM/TBST.