All lanes : Anti-DANRE clvs2 Antibody (C-term) at 1:2000 dilutionLane 1: Zebrafish whole cell lysateLane 2: ZF4 whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.