All lanes : Anti-CMA1 Antibody (N-Term) at 1:2000 dilutionLane 1: MCF-7 whole cell lysatesLane 2: A549 whole cell lysatesLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.