All lanes : Anti-Erythropoietin Antibody at 1:500 dilutionLane 1: Human liver lysateLane 2: HepG2 whole cell lysateLane 3: Mouse kidney lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.