All lanes : Anti-VSIG8 Antibody (C-term) at 1:2000 dilutionLane 1: RD whole cell lysateLane 2: U-87 MG whole cell lysateLysates/proteins at 20 �g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDaBlocking/Dilution buffer: 5% NFDM/TBST.