Detection of endogenous PKC theta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with PKCtheta antibody (Clone 1C2) at 0.5 ug/ml for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB-231 Lane 7: T47D