Detection of endogenous yes Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab yes-2B8 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: A431; lane 2: SW480; lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA231; lane 7: T47D