Detection of Endogenous Scramblase 1. Whole cell lysates of serum starved tumor cells were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Scram1-1E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK-293 Lane 4: SY5Y Lane 5: Jurkat Lane 6: U937 Lane 7: K562 Lane 8: THP