Applications: | ELISA, WB, FACS, ICC/IF |
Species Reactivity: | Human |
Isotype: | Mouse IgG1 kappa |
Immunogen: | Recombinant human Hsp27 (1-205aa) purified from E. coli |
Purification: | By protein-G affinity chromatography |
Conjugation: | Unconjugated |
Description: | Hsp27 (also known as the Estrogen-Regulated 24k protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress including elevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases including MAPkAP kinase 2 and the stress-activated protein kinase SAPk2 (p38). Hsp27 forms a multimeric complex within cells, and its primary chaperone function, like other small Hsps, appears to be the stabilization of denatured or aggregated proteins in a refolding competent state. |
Further Information: | The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:50. |
Synonym: | Heat shock 27 kDa protein, Hspb1, Hsp25, Hsp27, Hsp27, HspB1, Heat shock 27 kDa protein, HSP 27, Growth-related 25 kDa protein, P25, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein 28 kDa heat shock protein, CMT2F, DKFZp586P1322, Estrogen regulated 24 kDa protein, at shock 25kDa protein 1, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat Shock Protein27, HS.76067, Hsp 28, Hsp B1, Hsp28, HspB1, SRP27, Stress responsive protein 27. |
Ascension Number: | P04792 |
NCBI EntrezGene: | NP_001531 |
Shipping Conditions: | Ship on cold packs |
Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Usage: | Research use only |