| Applications: | ELISA, WB, IHC |
| Species Reactivity: | Human |
| Isotype: | Mouse IgG2b kappa |
| Immunogen: | Recombinant human HMGB1 (1-215aa) purified from High Five (Trichoplusia ni) insect cells. |
| Purification: | By protein-G affinity chromatography |
| Conjugation: | Unconjugated |
| Description: | High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. |
| Further Information: | The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~300. Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:200. |
| Synonym: | High-mobility group box1, HMG1, HMG3, SBP-1, High-mobility group box1, HMGB1, High-mobility group box1, high-mobility group (nonhistone chromosomal) protein 1, Sulfoglucuronyl carbohydrate binding protein, Amphoterin, high mobility group protein 1, DKFZp686A04236 |
| Ascension Number: | P09429 |
| NCBI EntrezGene: | NP_002119 |
| Shipping Conditions: | Ship on cold packs |
| Storage: | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
| Usage: | Research use only |
