Ordering Details
Cat Number:	AF6330-200ul
Size:	200uL
Concentration:	1mg/mL
Price:	£269.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

SOX9, Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:	Human, Mouse, Rat, Monkey
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human SOX9, corresponding to a region within the internal amino acids.
Purification:	The antiserum was purified by peptide affinity chromatography using SulfoLink� Coupling Resin (Thermo Fisher Scientific).
Conjugation:	Unconjugated
MW:	56kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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Immunohistochemical analysis of paraffin-embedded human brain tissue using SOX9 Antibody

Western blot analysis of extracts from various samples, using SOX9 Antibody. Lane 1: COS-7 treated with blocking peptide; Lane 2: COS-7; Lane 3: Hela; Lane 4: B16F10; Lane 5: HepG2.

AF6330 staining Hep G2 cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.