Immunohistochemical analysis of paraffin-embedded human brain tissue using SOX9 Antibody
Western blot analysis of extracts from various samples, using SOX9 Antibody. Lane 1: COS-7 treated with blocking peptide; Lane 2: COS-7; Lane 3: Hela; Lane 4: B16F10; Lane 5: HepG2.
AF6330 staining Hep G2 cells by IF/ICC.The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C.The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.