AF5390 staining HepG2 by IF/ICC.
AF5390 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
Western blot analysis of extracts from B16F10, using MEF2C Antibody.The lane on the left was treated with blocking peptide.