AF2016 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis of extracts of various tissue sample,using Phospho-GSK3 beta (Ser9) Antibody.
AF2016 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.