Ordering Details
Cat Number:	AF2006-200ul
Size:	200uL
Concentration:	1mg/mL
Price:	£269.00
Quantity:
Shipping:	£12.00

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Data Sheet

MSDS

p-NF-kB p65 [Ser536], Rabbit Polyclonal Antibody

Partner: Affinity Biosciences

Applications:	WB, IHC, IF/ICC, IP, ELISA
Species Reactivity:	Human, Mouse, Rat, Monkey
Isotype:	Rabbit IgG
Immunogen:	A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Ser536.
Purification:	The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Conjugation:	Unconjugated
MW:	65 kDa
Shipping Conditions:	Ship on cold packs
Storage:	Store at -20 C. Stable for 12 months from date of receipt.
Usage:	Research use only

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AF2006 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.

AF2006 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.