AF0825 at 1/100 staining human Rectum tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
AF0825 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37C.
Western blot analysis on HuvEc cell lysate using Phospho-APC(Ser2054) Antibody,The lane on the left is treated with the antigen-specific peptide.
