AF0155 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed.
Western blot analysis on Hela cell lysate using ERK1/2 Antibody
AF0155 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37C.The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37C.