Ordering Details
Cat Number:	ABT2040-2
Size:	200uL
Concentration:	1 mg/ml
Price:	£109.00
Quantity:
Shipping:	£12.00

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MSDS

HA Tag [4F6] Agarose, Mouse Monoclonal Antibody

Partner: Abbkine Scientific

Applications:	WB, IF, IP
Dilutions:	Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:2000), IP (1:400).
Isotype:	Mouse IgG
Immunogen:	Synthetic Peptide
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Description:	Featured Anti-HA Tag Mouse Monoclonal Antibody (4F6), specially designed for your taged protein quantification and localization.
Further Information:	Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
Shipping Conditions:	Ship on cold packs
Storage:	Stable for one year at -20 C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Usage:	Research use only

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A02040-if.jpg&&Fig.1. Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI.|||A02040-wb.jpg&&Fig.2.IP (1:400)-WB (1:10000) analysis of HA fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with HA-tag protein (lane B); IP untransfected 293 cell lysate with Anti HA tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D), or with Anti HA tag mAb (lane E), and IP transfected 293 without both normal Mouse IgG and HA tag mAb (lane F).